Blood parameter changes in lead acetate-exposed mice as a result of leonardite and lignite exposure

Abstract

The study aimed to research in vivo changes in the hematological and biochemical parameters of laboratory rats'
blood when they were continuously exposed to a moderate dose of lead acetate while receiving humic feed addi-
tives made of leonardite and lignite. Two-month-old white rats were used to assess the detoxifying properties
of the humic compounds leonardite and lignite. The analog pair approach was used to create four groups of eight
animals from 32 male laboratory rats for the study. Rats received a pre-meal injection of lead acetate at a dose
of 7 mg/100 g of animal weight (1/110 LD50) using a veterinary feeding needle. By creating solutions from
lignite and leonardite at a dosage of 18 and 25 mg/kg depending on the active ingredient, humic feed additives
were supplied to animals. It has been demonstrated that lignite- and leonardite-based feed additives may affect
the morphological parameters of lead acetate-treated rats' blood, including hemoglobin, hematocrit, erythrocyte,
and platelet count. These parameters were very near to the levels of the intact rats, which suggests that these
humic feed additives may have an anti-anemic impact. The effects of feed additives from leonardite and lignite
on labora- tory rats in groups that were also exposed to a toxic agent for all 21 days of the experiment led to the
normaliza- tion of markers of the state of protein metabolism in the group of intact animals (serum protein,
albumins, urea, creatinine), particularly activity of the enzyme’s aspartate aminotransferase and alanine
aminotransferase. As one of the most crucial indicators of lipid metabolism together with cholesterol, the
concentration of triglycerides was slightly lower in the humic substances treated groups than in the intact animals.
It was discovered that using a feed supplement made from leonardite in a dosage of 18 mg/kg, which contained
more fulvic acids than lignite, led to more favorable blood test results in the research group.