Development and Validation o fa GliadinInduced Intestinal Enteropathy Rat Model of Non-Celiac Gluten Sensitivity

Abstract

Ingestion of gluten-containing foods has been linked to a condition known as non-celiac gluten sensitivity (NCGS). Here, we create and verify a rat model of NCGS. The experimental group received 0.02 M acetic acid solution while the control group received 1.5 mg/g of body weight of gliadin in acetic acid solution. It was administered intragastrically through gavage to rats beginning on postnatal day 2 and continuing for a total of six weeks, three times per week. Changes in body weight, intestinal permeability, histology, proinflammatory cytokines, and IgG antibodies against gliadin (AGA).A lactulose/mannitol solution (500/250 mg/kg respectively) was administered 24 hours before sacrifice, and urine was collected to determine intestinal permeability. Small intestines were obtained, fixed, and hematoxylin and eosin stained for histological analysis. Breast cancer resistance protein (ABCG2) and P-glycoprotein (MDR1a) uptake transporter gene expression in the intestine was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Total anti-gliadin antibodies (AGA), AGA-IgA, AGA-IgM, and pro-inflammatory cytokines were measured in the blood samples taken.